bd40bc7c7a pp.3613363. Metaplastic cells often stain both green and pink at once. EA-36, EA-50, EA-65. The target structures are staine d orange in different intensities. The unmordanted haematein may be responsible for the yellow color imparted to glycogen.
Orange G Stain 3 minutes 12. Step Reagent Time 1. Unsourced material may be challenged and removed. Superficial cells are orange to pink, and intermediate and parabasal cells are turquoise green to blue. OBJECTIVES OF PAPANICOLAOU STAIN. 95% Alcohol 2 minutes 18. Orange Green 6 : This is the first acidic counterstain (cytoplasmic stain) which stains matured and keratinized cells. Mount in D.P.X . Pap stain is not fully standardized; it comes in several versions, subtly differing in the exact dyes used, their ratios, and timing of the process.